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Only two direct targets of the sex determination pathway that influence cell fate specification to produce hermaphrodite-specific cell fates have been identified.
Thus a major challenge will be to learn additional mechanisms by which the sex determination pathway interacts with signaling pathways and other cell fate specification genes to generate hermaphrodite-specific cell fates., hermaphrodite and male.
Genetic and molecular studies have identified many genes required for male development, most of which also function in the hermaphrodite.
Cell-cell interactions play a role in patterning all three of the generative tissues.
Male-specific neurons, including sensory neurons of the rays, hook, post-cloacal sensilla, and spicules, differentiate at the end of the last larval stage and send out axons to make connections into the existing neuropil, greatly enlarging the posterior ganglia.
The hindgut is highly differentiated to accommodate the spicules and the joining of the reproductive tract to the cloaca.
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Kainé is a companion and tritagonist of Nie R, who ends up accompanying Nier after he helps her kill Hook.
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